| Congresso Brasileiro de Microbiologia 2023 | Resumo: 1199-1 | ||||
Resumo:Endophytic fungi are microorganisms that live inside plant tissues throughout their life or during a specific period of their life cycles without causing visible damage or morphological harm, and in some cases, they can provide benefits to the hosts. Due to this characteristic, this group of microorganisms has aroused interest among researchers due to its potential application in the development of bio-inputs for agriculture. Among the mechanisms that aid in plant development are increased tolerance to salts, water scarcity, high temperatures, and disease control. In view of this, the present study aimed to characterize the potential as a bioinoculant of a Penicillium citrinum strain, belonging to the microorganisms collection of UTFPR Campus Toledo. For this purpose, in vitro tests were conducted to evaluate the ability to solubilize phosphate in PVK medium (Pikovskaya’s agar) and the production of IAA (indole acetic acid) in liquid culture, using YM medium supplemented or not with tryptophan. The production of volatile organic compounds (VOCs) with effects on plant growth was evaluated by co-culturing the microbial strain and watercress seeds in MS medium, followed by the evaluation of root growth. All tests were conducted in the absence of light for seven days in triplicate, and the data were subjected to ANOVA and Tukey's test at 95%.The strain was capable of solubilizing phosphate and producing auxin, both in the tryptophan supplemented and non-supplemented medium, reaching concentrations of 72.20 and 56.74 µg/mL, respectively. However, there was no statistical difference between the treatments. The presence of the fungus promoted 59% increase in plant root growth compared to the control group, demonstrating that the studied strain produces volatile compounds capable of promoting plant growth through indirect contact with the plant. Therefore, the strain shows potential for use in the development of bioinoculants, and further greenhouse tests could confirm its efficiency.
Palavras-chave: Auxin, Bioinoculants, Endophytic fungi, Phosphate solubilization Agęncia de fomento:Universidade Tecnológica Federal do Paraná | |||||